Supplementary Material for: Proteome and Transcriptome Reveal Involvement of Heat Shock Proteins and Indoleacetic Acid Metabolism Process in <b><i>Lentinula Edodes</i></b> Thermotolerance
2018-11-01T12:46:36Z (GMT) by
<b><i>Background/Aims:</i></b> Heat stress could cause huge losses for <i>Lentinula edodes</i> in China and other Asian cultivation areas. Yet our understanding of mechanism how to defend to heat stress is incomplete. <b><i>Methods:</i></b> Using heat-tolerant and heat-sensitive strains of <i>L. edodes</i>, we reported a combined proteome and transcriptome analysis of <i>L. edodes</i> response to 40 °C heat stress for 24 h. Meanwhile, the effect of <i>LeDnaJ</i> on the thermotolerance and IAA (indoleacetic acid) biosynthesis in <i>L. edodes</i> was analyzed via the over-expression method. <b><i>Results:</i></b> The proteome results revealed that HSPs (heat shock proteins) such as Hsp40 (DnaJ), Hsp70, Hsp90 and key enzymes involved in tryptophan and IAA metabolism process LeTrpE, LeTrpD, LeTam-1, LeYUCCA were more highly expressed in S606 than in YS3357, demonstrating that HSPs and tryptophan as well as IAA metabolism pathway should play an important role in thermotolerance. Over-expression of <i>LeDnaJ</i> gene in S606 strains showed better tolerance to heat stress. It was also documented that intracellular IAA accumulation of S606 (8-fold up) was more than YS3357 (2-fold up), and exogenous IAA enhanced <i>L. edodes</i> tolerance to heat stress. <b><i>Conclusion:</i></b> Our data support the interest of LeTrpE, LeDnaJ, tryptophan and IAA could play a pivotal role in enhancing organism thermotolerance.