Supplementary Material for: Molecular and Cytological Comparisons of Chromosomes 7el<sub>1</sub>, 7el<sub>2</sub>, 7E<sup>e</sup>, and 7E<sup>i</sup> Derived from <b><i>Thinopyrum</i></b>

<i>Thinopyrum</i> chromosomes 7el<sub>1</sub>, 7el<sub>2</sub>, 7E<sup>e</sup>, and 7E<sup>i</sup>, homoeologous to group 7 chromosomes of common wheat <i>(Triticum aestivum)</i>, were determined to have many useful agronomical traits for wheat improvement. To analyze the genetic relationships among the 4 <i>Thinopyrum</i> 7E chromosomes, the conserved orthologous set markers, genomic in situ hybridization (GISH), and meiotic chromosome pairing were used in this study. The unweighted pair-group method with arithmetical averages (UPGMA) analysis indicated that 7el<sub>1</sub>, derived from <i>T. ponticum</i>, and 7E<sup>i</sup>, derived from <i>T. intermedium</i>, were the most closely related. 7el<sub>2</sub>, derived from <i>T. ponticum</i>, was relatively distant from the 7el<sub>1</sub>-7E<sup>i</sup> complex. While 7E<sup>e</sup>, derived from <i>T. elongatum</i>, was more distantly related to 7el<sub>1</sub>, 7el<sub>2</sub>, and 7E<sup>i</sup>. This is the first report showing that 7el<sub>1</sub> and 7E<sup>i</sup> may be similar, which could be explained by the similar chromosome signal distribution revealed by GISH as well as UPGMA analysis revealed by both molecular markers and the highest frequency of meiotic pairing. The newly developed genome-specific molecular markers may be useful for marker-assisted selection of <i>Lr19</i>, <i>Bdv3</i>, and <i>Fhblop</i>.