Supplementary Material for: High Amount of Organic Matter during Caries Formation Reduces Remineralization and Resin Infiltration of Enamel Caries

The amount of organic material in the cariogenic environment correlates with the amount of organic material incorporated in carious enamel. The incorporated organic material may be expected to reduce the pore volumes available for remineralization and resin infiltration, but these expected outcomes have not yet been quantified. We tested the effect of the amount of organic content in the cariogenic agent on remineralization and the resin-occluded pore volume in artificial subsurface enamel caries. An acid gel (organic-rich; G1) and an aqueous solution (organic-poor; G2) were used to induce subsurface lesions in human enamel. Undemineralized histological sections were prepared, microradiographed, and then submitted to resin infiltration in vitro. The enamel component volumes (mineral, organic, remineralizable [total water volume], loosely and firmly bound water volumes, and resin-occluded volume) were measured (by microradiography and polarizing microscopy) at histological sites (<i>n</i> = 38, G1; <i>n</i> = 34, G2). The main outcomes were the differences between the experimental and the predicted volumes (Δremineralizable and Δresin-occluded volumes). Resin infiltration was confirmed by confocal scanning laser microscopy. Compared to G2, G1 presented more incorporated organic volume and lower Δremineralizable volume (<i>p</i> = 0.003; Hedges <i>g</i> = 0.66; power = 0.87), a lower increase in loosely bound water volume (<i>p</i> = 0.0013; Hedges <i>g</i> = 0.74; power = 0.93), a lower remineralization volume in the surface layer (<i>p</i> = 0.017; Hedges <i>g</i> = 0.68; power = 0.8), and a lower Δresin-occluded volume (<i>p</i> = 0.0015; Hedges <i>g</i> = 0.73; power = 0.92). In conclusion, the higher amount of organic matter in the cariogenic gel negatively affected remineralization and the resin-occluded volume in subsurface lesions.