Supplementary Material for: Head-Out Spirometry Accurately Monitors the Course of <i>Pseudomonas aeruginosa</i> Lung Infection in Mice

<i>Background:</i> Classic infection models in rodents use lethal doses of bacteria as inocula, thus creating models which are rarely comparable to the clinical situation. Moreover, single time-point evaluation requires killing of the animals, necessitating large numbers of animals. Longitudinal parameters such as temperature appear to have a relatively low accuracy. Spirometry might be an accurate method to assess the course of a bacterial lung infection without the necessity to sacrifice the animals. <i>Objectives:</i> We measured lung function in C57BL/6JZtm mice following intratracheal infection with <i>Pseudomonas aeruginosa</i> and compared it to physiological parameters and lung histology. <i>Methods:</i> Head-out spirometry measuring 14 parameters was performed on C57BL6/J mice for eight days following a <i>P. aerugino</i>sa lung infection. Additionally rectal temperature, body weight and condition were assessed together with histological data and bacteriological clearance. <i>Results:</i> Several spirometric parameters were significantly altered for more than 72 h after inoculation, which was four times longer than observed alterations in physiological parameters such as temperature. Volume (amount of air inspired) decreased more than seven-fold within 6 h after inoculation and required 72 h to recover, rendering it the most sensitive spirometric parameter investigated. Spirometric and histological data correlated well. <i>Conclusions:</i> Our findings suggest that non-invasive head-out spirometry is a reliable and highly sensitive method to longitudinally assess the course of bacterial lung infections.