Supplementary Material for: Genomic structure and transcript variants of the bovine <i>DAZL</i> gene

The Deleted in AZoospermia Like <i>(DAZL)</i> gene is a member of the DAZ family and encodes an RNA-binding protein that is expressed in prenatal and postnatal germ cells of males and females. In the human, there are five highly-related members in the DAZ family, four <i>(DAZ1–4)</i> on the Y chromosome and one <i>(DAZL)</i> on an autosome (HSA3). Mutations in these genes have been linked to severe spermatogenic failure and infertility in men. In the present study, we have cloned and characterized the bovine <i>DAZL</i> (b<i>DAZL</i>) gene. The full-length b<i>DAZL</i> cDNA is predicted to encode a protein of 295 amino acids with an RNA recognition motif. The deduced protein sequence of bDAZL is 96 and 97% similar to human and mouse DAZL, respectively. Fluorescence in situ hybridization (FISH) maps b<i>DAZL</i> to the distal region on BTA1q. The b<i>DAZL</i> gene consists of 11 exons and 10 introns. A b<i>DAZL</i> pseudogene was identified on BTA16. Expression analysis of b<i>DAZL</i> in 13 different tissues by RT-PCR shows that two transcripts, variant 1 (2,996 bp) and variant 2 (1,373 bp), of the b<i>DAZL</i> gene are detected only in testis mRNA. The variants probably result from alternative RNA splicing as variant 1 contains an additional 1,623-bp insertion in the 3′ UTR. Our results lay the groundwork for possible single nucleotide polymorphism (SNP) and functional studies of the <i>DAZL</i> gene in cattle.




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