Supplementary Material for: Determining Stroke Onset Time Using Quantitative MRI: High Accuracy, Sensitivity and Specificity Obtained from Magnetic Resonance Relaxation Times

Many ischaemic stroke patients are ineligible for thrombolytic therapy due to unknown onset time. Quantitative MRI (qMRI) is a potential surrogate for stroke timing. Rats were subjected to permanent middle cerebral artery occlusion and qMRI parameters including hemispheric differences in apparent diffusion coefficient, T2-weighted signal intensities, T1 and T2 relaxation times (qT1, qT2) and f1, f2 and Voverlapwere measured at hourly intervals at 4.7 or 9.4 T. Accuracy and sensitivity for identifying strokes scanned within and beyond 3 h of onset was determined. Accuracy for Voverlap, f2 and qT2 (>90%) was significantly higher than other parameters. At a specificity of 1, sensitivity was highest for Voverlap (0.90) and f2 (0.80), indicating promise of these qMRI indices in the clinical assessment of stroke onset time.