Supplementary Material for: Antibiotic Susceptibility and Molecular Characterization of Escherichia coli O157 Isolates from Urinary Tract Infections

<p><b><i>Background:</i></b><i>Escherichia coli O157</i> is an important food-borne pathogen that can cause diarrhoea, hemorrhagic colitis, and hemolytic uraemic syndrome. Enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and multilocus sequence typing (MLST) are good methods for molecular typing and the extensive use of antibiotics is a contributing factor to the increasing incidence of antimicrobial-resistant for these strains. <b><i>Objectives:</i></b> The aim of this study was to determine the prevalence, antimicrobial resistance, and genetic diversity of <i>E. coli O157</i> based on the prevalence of urinary tract infection (UTI) in Hubei, China. <b><i>Results:</i></b> We obtained 23 (8.07%) <i>E. coli O157</i> isolates from 285 UTI patients in Hubei, China. All isolates were subjected to antibiotic susceptibility analysis, and molecular typing was performed using ERIC-PCR and MLST. Antimicrobial susceptibility results indicated that most strains were resistant to penicillin (95.65%), chloramphenicol (73.91%), and ampicillin (69.57%). All isolates were discovered to be multiresistant (resistance to more than 3 antibiotics). Genetic variability analysis showed that all of the isolates were grouped into 4 clusters both by ERIC-PCR and MLST. <b><i>Conclusion:</i></b> Our findings demonstrated the presence of <i>E. coli O157</i> in UTIs, provided insights into the dissemination of antibiotic-resistant strains, and improved our knowledge of <i>E. coli O157</i> risk assessment in UTIs.</p>