Supplementary Material for: A Six-SNP Haplotype of <i>ADAM33 </i>Is Associated with Asthma in a Population of Cartagena, Colombia
2009-11-24T00:00:00Z (GMT) by
<i>Background:</i> A disintegrin and metalloprotein-33 <i>(ADAM33)</i> participates in the bronchial remodeling process in asthma, and genetic analyses pointed it out as a candidate gene in asthma. <i>Methods:</i> To analyze the association between <i>ADAM33</i> and asthma and total and mite-specific IgE levels in a population of the Caribbean Coast of Colombia, we genotyped 6 single-nucleotide polymorphisms of <i>ADAM33</i> in 429 asthmatics, 401 controls and 116 family trios using fluorogenic probes. Total and specific IgE against <i>Blomia tropicalis </i>and <i>Dermatophagoides pteronyssinus </i>were determined by ELISA. Case-control and family-based analyses were performed. Case-control association analyses were corrected by population stratification using a set of 52 ancestry-informative markers. <i>Results:</i> Eight common haplotypes were identified; among them, H4 (GCAGGG) was associated with asthma in the family group (Z score: –2.049, p = 0.04). We also found an association between the TT genotype of ST+7 and asthma in the case-control study (p = 0.05) that disappeared after correcting for multiple testing. In the family-based analysis, this genotype was a risk factor for asthma (p = 0.01), high total IgE (Z score: 2.546, p = 0.01) and high specific IgE against<i> B. tropicalis</i> (p = 0.02) and <i>D. pteronyssinus</i> (Z score: 2.414, p = 0.01). V4 was associated with specific IgE against <i>B. tropicalis</i> (p = 0.03); T2 with asthma (p = 0.03), high total IgE (p = 0.02) and IgE against D. <i>pteronyssinus</i> (p = 0.03) and T1 with high total IgE (p = 0.04). None of these associations was maintained after correction for multiple testing. <i>Conclusions:</i> Our findings suggest a relevant role of <i>ADAM33 </i>in thepathogenesis of asthma in this population.