Supplementary Figures 1 and 2 from Wnt controls the medial–lateral subdivision of the Drosophila head

Figure 1: Attenuation of the Wnt signaling pathway in the prospective head vertex causes the development of ectopic compound eyes. Confocal images of imaginal discs of the indicated genotypes (A-C). The boxed regions are shown at higher magnification in (A’-C’). Discs are stained for the retinal determination gene Eya (red) and the photoreceptor marker Elav (blue). Hth (homotorax, green), which marks the prospective head capsule and antenna (“a”), is used as a counterstain. In (A’-C’) merged, Eya and Elav channels are shown. Red arrows point to Elav-expressing photoreceptors within the ectopic eye tissue.; Figure 2: Ey and Otd are coexpressed in EAD and this coexpression might specifiy periorbital cuticle. (A,C,D) Dorsal views of adult heads from wildtype (A), oc2>ey (C) and optix>otd individuals (D). oc2-GAL4 is a dorsal head specific driver, while optix2/3-GAL4 (“optix”) drives expression in the undifferentiated region of the eye primordium. The periorbital cuticle (red double-headed arrow) is extended in oc2>ey heads. Similarly, when otd is expressed in the developing eye, head-like cuticle develops at the expense of the compound eye (CE). (B) Confocal image showing the boundary between the Otd- and Ey-expressing regions in the eye-antennal disc of a late L3 wild type larva. There is a region of overlap between Otd and Ey domains that corresponds to the prospective periocular region (bracketed). Compound eye (CE) is pseudocolored in red.