Supplementary Figure S3. Apoptosis correlates with hypoxic cores in larger spheroids. from Measurement of oxygen tension within mesenchymal stem cell spheroids

(A) Annexin V visibly stained larger spheroids, and a statistically significant apoptotic core appears beginning with spheroids containing 250,000 cells. Annexin V, red; DAPI, blue. (B) Quantification of apoptosis index, calculated by the area of positive Annexin V staining in the core relative to the periphery (n=3). Groups with no significance are linked by the same letters, while groups with significance do not share the same letters.