figshare
Browse
rsif20160851_si_002.pdf (13.89 MB)

Supplementary Figure S2. Large spheroids exhibit a hypoxic core. from Measurement of oxygen tension within mesenchymal stem cell spheroids

Download (13.89 MB)
journal contribution
posted on 2017-01-27, 11:08 authored by Kaitlin C. Murphy, Ben P. Hung, Stephen Browne-Bourne, Dejie Zhou, Jessica Yeung, Damian C. Genetos, J. Kent Leach
(A) Spheroids formed from 100,000, 250,000, and 500,000 MSCs per spheroid exhibited positive pimonidazole staining, suggesting the presence of hypoxia throughout the aggregate. (B) Quantification of hypoxia index, calculated by the area of positive pimonidazole staining in the core relative to the periphery (n=3). Pimonidazole, green; DAPI, blue. Groups with no significance are linked by the same letters, while groups with significance do not share the same letters.

History

Usage metrics

    Journal of the Royal Society Interface

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC