Supplementary Figure S2. Large spheroids exhibit a hypoxic core. from Measurement of oxygen tension within mesenchymal stem cell spheroids

(A) Spheroids formed from 100,000, 250,000, and 500,000 MSCs per spheroid exhibited positive pimonidazole staining, suggesting the presence of hypoxia throughout the aggregate. (B) Quantification of hypoxia index, calculated by the area of positive pimonidazole staining in the core relative to the periphery (n=3). Pimonidazole, green; DAPI, blue. Groups with no significance are linked by the same letters, while groups with significance do not share the same letters.