Supplemental Material for Zhang et al., 2018

<div>Figure S1 shows that a donor-free transformation using a gene-targeting sgRNA insert resulted in a substantially lower transformation efficiency than a control transformation using a control sgRNA insert examples of spindle categories.</div><div>Figure S2 shows the effects of different amounts of the gapped plasmid and the sgRNA insert on rpl42-P56Q knock-in when using the split-ura4 system.</div><div>Figure S3 shows the effects of different types of donor DNA on rpl42-P56Q knock-in when using the split-ura4 system.</div><div>Figure S4 shows the effects of different amounts of donor DNA on rpl42-P56Q knock-in when using the split-ura4 system.</div><div>Figure S5 shows that tor2-L2048S mutants generated using the split-ura4 system behaved as expected.</div><div>Figure S6 shows that cdc25-C532Y mutants generated using the split-ura4 system behaved as expected.</div><div>Figure S7 shows that mECitrine-Ypt7 strains generated using the split-ura4 system showed the expected localization of mECitrine fluorescence on the vacuole membrane.</div><div><br></div><div>Table S1 lists oligos used for plasmid construction.</div><div>Table S2 lists oligos used as donors or as primers for donor amplification.</div><div>Table S3 shows the results of PCR and Sanger sequencing analysis of Ura+ transformants obtained in the temperature-sensitive (ts) mutation knock-in experiments.</div><div><br></div><div>File S1 is the plasmid map file in GenBank format for pDB4279.</div><div>File S2 is the plasmid map file in GenBank format for pDB4280.<br></div><div>File S3 is the plasmid map file in GenBank format for pDB4281.<br></div><div>File S4 is the plasmid map file in GenBank format for pDB4282.<br></div><div>File S5 is the plasmid map file in GenBank format for pDB4283.<br></div>