Supplemental Material for Kweon et al., 2018

Figure S1 sra^KO mutant flies display normal morphology in axonal projections from PDF-expressing circadian pacemaker neurons.

Figure S2 sra^KO mutation decreases PER and TIM levels in PDF-expressing circadian pacemaker neurons. Adult fly brains were dissected at ZT23 in LD cycles.

Figure S3 A transgenic reporter for sra promoter activity (sra-Gal4) is expressed in various brain regions of adult fly.

Figure S4 sra depletion in PDF-expressing clock neurons is sufficient to dampen rhythm amplitude in free-running circadian locomotor behaviors.

Figure S5 Conditional depletion of sra in PDF-expressing neurons causes poor rhythmicity in free-running locomotor behaviors.

Figure S6 Genomic deletion of each calcineurin subunit weakens rhythm amplitude in free-running circadian locomotor behaviors.

Figure S7 Pharmacological inhibition of calcineurin reduces the steady-state levels of PER and TIM, but not CLK proteins, in Drosophila S2 cells.

Table S1 sra mutant flies display poor rhythmicity in free-running locomotor behaviors in DD.

Table S2 sra depletion in PDF-expressing clock neurons is sufficient to dampen rhythm amplitude in free-running circadian locomotor behaviors.

Table S3 Conditional depletion of sra in PDF-expressing neurons dampens free-running circadian locomotor rhythms.

Table S4 sra overexpression in circadian pacemaker neurons disrupts free-running locomotor rhythms in DD.

Table S5 Genomic deletion of each calcineurin subunit weakens rhythm amplitude in free-running circadian locomotor behaviors.

Table S6 A constitutively active form of Pp2B-14D disrupts free-running circadian behaviors in a dose-dependent manner.

Table S7 sra genetically suppresses Pp2B-14D^ACT to sustain circadian locomotor rhythms.

Table S8 Oral administration of cyclosporine A (CsA) lengthens circadian periods in free-running locomotor rhythms of wild-type flies.