Supplemental Material for Kaplan et al., 2018

<p>Complete normalized expression data set. Raw data is available upon request. </p><p><br></p><p><strong> <br></strong></p><p>Supp Data File 1. Complete statistics for regulation of all genes during starvation and recovery by <i>daf-16</i> and temperature.</p><p></p><p><br></p>Supp Data File 2. Complete normalized expression data set. Raw data is available upon request.<p><br></p><p></p><p><strong> </strong>Figure S1 - complementing Fig. 2. Insulin-like signaling regulates expression of genes comprising the insulin-like signaling pathway during L1 starvation. Transcript abundance (arbitrary units) is plotted during L1 starvation at three different temperatures in five different genotypes with various levels of insulin-like signaling activity. In addition to <i>daf-2</i>/InsR and components of the PI3K pathway, the transcriptional effectors of signaling, <i>daf-16</i>/FoxO and <i>skn-1</i>/Nrf, are plotted as well as the known DAF-16 target <i>sod-3</i>. Note that <i>daf-16 </i>expression was not detected in <i>daf-16 </i>mutants. Each gene plotted was significantly affected by <i>daf-16</i> (see Tables 1, S1 and Supp. Data). Error bars reflect the SEM of two or three biological replicates. </p><p>Figure S2 - complementing Fig. 3. Insulin-like signaling and temperature regulate expression of the majority of insulin-like genes during L1 starvation. Transcript abundance (arbitrary units) is plotted during L1 starvation at three different temperatures in five different genotypes with various levels of insulin-like signaling activity. Of 28 reliably detected insulin-like genes, 25 were significantly affected by <i>daf-16</i> and are plotted (same genes as in Fig. 3). 21 were significantly affected by temperature (all but <i>ins-2, 9, 12, 16, 21, 29, 34</i>). <i>ins-10 </i>was affected by temperature but not <i>daf-16 </i>and is not plotted here (see Tables 1, S1 and Supp. Data). Error bars reflect the SEM of two or three biological replicates.</p><p></p><p><strong> </strong>Table S1. Summary of genes whose expression is affected by temperature during L1 starvation. </p><p><br></p><p></p><p></p><p></p><p><br></p><p></p><p></p><p><br></p><p></p><p><br></p><p><br></p><p><br></p><p><br></p><p><br></p><p> <br></p><p><br></p><p><br></p><p> <br></p><p><br></p><p> <br></p><p><br></p><p><br></p>