Supplemental Material for Glaser-Schmitt, Zečić, and Parsch, 2018

Figure S1 shows the distribution of the coefficient of variation (CV) for all genes between biological replicates of each genotype. Figure S2 shows the extended haplotype analysis of the tested P450 upstream regions and their 50 kb surrounding regions. Figure S3 shows the ratio of African-to-European expression of the cytochrome P450 genes of interest in the Malpighian tubule. Table S1 contains the tested upstream region coordinates and PCR primers. Table S2 shows the additional sequencing primers used for the P450 upstream regions. Table S3 shows the sequence depth of the candidate P450 genes. Table S4 contains the RNA-seq read count data for each genotype. Table S5 shows the overlap between In(2L)t-affected genes and differentially expressed genes in comparisons involving strain S26. Table S6 shows the TFBS models with predicted differential binding between the tested S58 and Z418 upstream regions. Table S7 lists the segregating sites in the tested P450 upstream regions and their frequencies in a Dutch and a Zambian population. Table S8 shows summary statistics and genetic differentiation of tested upstream regions in a Dutch and a Zambian population. Table S9 shows summary statistics and genetic differentiation of 50 kb surrounding the tested P450 upstream regions in a Dutch and a Zambian population.