Supplemental Material for Edskes et al., 2018
2018-05-16T16:24:15Z (GMT) by
Supplemental Methods_figures_tables.pdf - Provides a detailed description of the culture conditions, induction of <i>Hermes</i> transposition, selection of colonies carrying a transposition, extraction of cellular DNA, PCR amplification and isolation of the junction points between transposon and chromosomal insertion site, next-generation sequencing of these sites, and analysis of the data by visual display and by counting insertions per open reading frame. <br><br> Exon Intron Counts.xlsx - Gives the insertions in every yeast open reading frame, distinguishing exons from introns where appropriate.<br><br>Sorted Hits.xlsx - Gives prominent hits sorted by functional group, “TY gag-pol Counts.xlsx comparing insertions in the Ty retrotransposons at different locations in the genome. <br><br>Count Insertions in ORFs and Introns.txt - The Python program used for counting insertions. <br><br>LUGsIGV-InsertDistributions.pptx - Is a slide show of insert distributions in each of 500 genes for which insertions were recovered more frequently in [ure-o] than in [URE3] cultures.