Supplemental Figure 2. CD147 is enriched at sites of L. monocytogenes cell-to-cell spreading in Caco-2 cells.

Caco-2 cells were infected with wild-type L. monocytogenes for 5 hours then fixed and stained with mouse monoclonal CD147-targeting antibodies (green), Alexa594-phalloidin (red) to visualize F-actin and DAPI (blue) to visualize DNA. CD147 was enriched at the plasma membrane surrounding membrane protrusions. The open arrowhead indicates the L. monocytogenes bacterium (blue). The solid arrowhead points to CD147 at the membrane protrusion. Scale bar is 5 μm.