Supplemental Figure 1 from Modulation of social space by dopamine in <i>Drosophila melanogaster,</i> but no effect on the avoidance of the <i>Drosophila</i> stress odorant

Increased social spacing in loss of function of VMAT males in large chambers. In these experiments, social spacing was performed on groups of 40 flies in large chambers (see text). A: A heterozygous P element insertion in VMAT - L(2)SH0459 - tested both in red eyed (w+;p/+) and orange eyed (w-;p/+) background (both in doted box) shows the same decrease in social space, significantly different from that of the red eyed wild-type background control Canton-S (Cs – in dark grey box), and from the non-social situation (in open box – see text; n= 2 to 3 repeats of 40 males, p < 0.0001; a#b p<0.003, a#c p<0.03, a#d p<0.007). B. Males with one UAS-VMAT RNAi under a TH-Gal 4 driver (n=4X40) show a trend to have increased social spacing compared to controls (CS n=4X40, TH-Gal/+ n=4X40, VMAT RNAi/+ n=3X40; p < 0.0001; letters indicate statically different groups a#b p<0.003, a#c p<0.0001, b#c p<0.009). C. Males with 2 copies of UAS-RNAi VMAT-A (Dopplex VMAT RNAi) under TH-Gal4 driver (doted box, n=3X40) displays the same trend as the P-element loss of function: increased social spacing, compared to its controls (TH-Gal/+ n=2X40, Dopplex VMAT RNAi/+ n=2X40, all in dark grey boxes). But it is not different from non-social flies (open box). p < 0.0001; a#b p<0.00001. D: Males overexpressing UAS-VMAT under a TH-Gal4 driver (n=6X40) have a slight but statistically significant decrease in social space compared to their controls (UAS-VMAT/+ and TH-Gal4/+, each n=2X40) in dark grey (p<0.02, a#b p<0.02).;