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Schematic of the pSK-Tel-Kan-Blast-186 vector used to silence the Srb1 gene in the wild-type G217B strain.

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posted on 2016-10-06, 17:50 authored by Juwen C. DuBois, A. George Smulian

Plasmid contains inverted copies of Srb1 separated by a lacZ fragment to produce an RNA hairpin.

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    Sterol Regulatory Element Binding ProteinHRGHistoplasma capsulatum sterolDimorphic Fungus Histoplasma capsulatumSrb 1 message levels increaseRNAantifungal azole drug itraconazoleelement binding proteincapsulatum hypoxia-responsive genesDNASrb 1 expressiongene encoding Srb 1SREBPSrb 1encode ergosterol biosynthetic enzymes

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