Stereoselective Bioreduction of Bulky-Bulky Ketones by a Novel ADH from Ralstonia sp.

Lavandera, Iván; Kern, Alexander; Ferreira-Silva, Bianca; Glieder, Anton; Wildeman, Stefaan de; Kroutil, Wolfgang

doi:10.1021/jo800849d.s001

jo800849d_si_001.pdf (116.46 kB)

Stereoselective Bioreduction of Bulky-Bulky Ketones by a Novel ADH from Ralstonia sp.

journal contribution

posted on 2008-08-01, 00:00 authored by Iván Lavandera, Alexander Kern, Bianca Ferreira-Silva, Anton Glieder, Stefaan de Wildeman, Wolfgang Kroutil

Ketones with two bulky substituents, named bulky-bulky ketones, as well as less sterically demanding ketones were successfully reduced to the corresponding optically highly enriched alcohols using a novel identified recombinant short-chain alcohol dehydrogenase RasADH from Ralstonia sp. DSM 6428 overexpressed in E. coli.