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Startup of Electromethanogenic Microbial Electrolysis Cells with Two Different Biomass Inocula for Biogas Upgrading
journal contribution
posted on 2017-09-07, 00:00 authored by Míriam Cerrillo, Marc Viñas, August BonmatíThe
performance and biomass enrichment of the biocathode of a pair
of lab-scale two-chambered microbial electrolysis cells (MEC) were
assessed for 95 days as a technology for upgrading the biogas produced
in anaerobic digesters, converting CO2 into CH4 through the electromethanogenic process. Two different inocula were
compared: (i) a mixture of biomass from the anode of a MEC and anaerobic
granular sludge (BC1); (ii) biomass enriched in a methanol-fed upflow
anaerobic sludge blanket reactor (UASB) (BC2). Quantitative and qualitative
microbial community assessment of the enrichment process on the biocathodes
was performed by means of high-throughput sequencing of 16S
rDNA- and 16S rRNA-based massive libraries
as well as RT-qPCR of 16S rRNA and mcrA genes. Although BC2 had a faster increase in current density than
BC1, there were no significant differences neither in the average
CH4 production (0.23 ± 0.01 and 0.22 ± 0.05 L
m–3 day–1 for BC1 and BC2, respectively)
nor in the cathodic methane recovery efficiency (65 ± 8% and
79 ± 17%, respectively). Independently from the origin of the
inoculum, total and active archaeal microbial community in both biocathodes
was dominated by hydrogenotrophic methanogenic archaea, especially
belonging to Methanobacteriaceae family (mainly Methanobrevibacter genus) (84–98% of both 16S rDNA and 16S rRNA relative abundance).
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Methanobacteriaceae familyMethanobrevibacter genuslab-scale two-chamberedBC 1Different Biomass InoculaBC 2biocathodeBiogas Upgradingcommunity assessmentbiomass enrichmenthydrogenotrophic methanogenic archaeaCO 2enrichment processhigh-throughput sequencing95 daysUASBelectromethanogenic processCH 4Electromethanogenic Microbial Electrolysis CellsMECmcrA geneselectrolysis cellsmethanol-fed upflow16 S rDNAsludge blanket reactor16 S rRNA
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