Spermatogenesis-inducing effects of BSAs purified by different methods.

<p><b>A</b>) GFP expression grade time-course in mouse testis tissues cultured with 4 BSA products. Asterisk indicates P<0.01. <b>B</b>) Histological appearance of testis tissues cultured for 20 days with each BSA. Typical pachytene-stage spermatocytes are indicated with arrows. Immunohistochemistry was performed with a pan-germ cell marker (Tra98, Red) and meiosis marker (Stra8, Green). <b>C</b>) GFP expression grade time-course for 41 days (corresponding to 46 dpp). All media in each group were converted to media containing AlbuMAX on culture day 20.</p>