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Spermatogenesis-inducing effects of BSAs purified by different methods.

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posted on 2018-02-12, 18:45 authored by Hiroyuki Sanjo, Mitsuru Komeya, Takuya Sato, Takeru Abe, Kumiko Katagiri, Hiroyuki Yamanaka, Yoko Ino, Noriaki Arakawa, Hisashi Hirano, Tatsuma Yao, Yuta Asayama, Akio Matsuhisa, Masahiro Yao, Takehiko Ogawa

A) GFP expression grade time-course in mouse testis tissues cultured with 4 BSA products. Asterisk indicates P<0.01. B) Histological appearance of testis tissues cultured for 20 days with each BSA. Typical pachytene-stage spermatocytes are indicated with arrows. Immunohistochemistry was performed with a pan-germ cell marker (Tra98, Red) and meiosis marker (Stra8, Green). C) GFP expression grade time-course for 41 days (corresponding to 46 dpp). All media in each group were converted to media containing AlbuMAX on culture day 20.

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