bm5b00955_si_001.pdf (323.36 kB)
Specific Adsorption via Peptide Tags: Oriented Grafting and Release of Growth Factors for Tissue Engineering
journal contribution
posted on 2015-11-09, 00:00 authored by Frederic Murschel, Aldo Zaimi, Samantha Noel, Mario Jolicoeur, Gregory De CrescenzoNumerous strategies have been proposed
to decorate biomaterials
with growth factors (GFs) for tissue engineering applications; their
practicability as clinical tools, however, remains uncertain. We previously
presented two complementary amphipathic peptides, namely, E5 and K5,
which could be utilized as tags to direct GF capture onto organic
materials via E5/K5 coiled-coil interactions. We here investigated
their potential as mediators of GF physical adsorption. Enzyme-linked
immunosorbent assays highlighted that both electrostatic and hydrophobic
interactions could contribute to the adsorption process, without interfering
with the peptides propensity for coiled-coil interactions. E5-tagged
vascular endothelial growth factor, in particular, was efficiently
adsorbed to poly(allylamine)-functionalized polystyrene, was maintained
in a bioactive state and was steadily liberated over several days
with little initial burst. This simple immobilization procedure was
successfully applied to poly(ethylene terephthalate) films. Altogether,
our data demonstrated that coil-tag-directed adsorption is a tunable,
versatile and straightforward strategy to decorate biomaterials with
GFs.