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Size-Dependent Programming of the Dynamic Range of Graphene Oxide–DNA Interaction-Based Ion Sensors
journal contribution
posted on 2014-04-15, 00:00 authored by Huan Zhang, Sisi Jia, Min Lv, Jiye Shi, Xiaolei Zuo, Shao Su, Lianhui Wang, Wei Huang, Chunhai Fan, Qing HuangGraphene oxide (GO)
is widely used in biosensors and bioimaging
because of its high quenching efficiency, facile chemical conjugation,
unique amphiphile property, and low cost for preparation. However,
the nanometer size effect of GO on GO–DNA interaction has long
been ignored and remains unknown. Here we examined the nanometer size
effect of GO on GO–DNA interactions. We concluded that GO of
∼200 nm (lateral nanometer size) possessed the highest fluorescence
quenching efficiency whereas GO of ∼40 nm demonstrated much
weaker ability to quench the fluorescence. We employed the nanometer
size effect of GO to program the dynamic ranges and sensitivity of
mercury sensors. Three dynamic ranges (1 to 40 nM, 1 to 15 nM, and
0.1 to 5 nM) were obtained with this size modulation. The sensitivity
(slope of titration curve) was programmed from 15.3 ± 1.27 nM–1 to 106.2 ± 3.96 nM–1.