Sirolimus significantly inhibited pH1N1 virus-induced mTOR pathway activation in vivo.

(A) Western blot analysis of p-mTOR, mTOR, p-S6K, S6K, p-S6RP, and S6RP expression in the lungs of different treatment groups at 5 dpi. (B) Expression of p-mTOR relative to mTOR, p-S6K relative to S6K, p-S6RP relative to S6RP, and the mTOR, S6K, and S6RP relative to β-actin in the different treatment groups. (C) Representative immunohistochemical images of p-mTOR and p-S6RP expression in the lungs of mice in the different treatment groups at 5 dpi. Scale bar = 200 μm, original magnification: ×100. (D) Quantitative analysis of p-mTOR- and p-S6RP-positive cells in the lungs of infected mice after the different treatments. Data are representative of two independent experiments and presented as mean ± SEM (n = 3 for each group). *, **, and *** represent p < 0.05, 0.01, and 0.001, respectively.