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Singlet Oxygen Generation by the Genetically Encoded Tag miniSOG
journal contribution
posted on 2016-02-19, 04:28 authored by Rubén Ruiz-González, Aitziber L. Cortajarena, Sara H. Mejias, Montserrat Agut, Santi Nonell, Cristina FlorsThe genetically encodable fluorescent
tag miniSOG is expected to
revolutionize correlative light- and electron microscopy due to its
ability to produce singlet oxygen upon light irradiation. The quantum
yield of this process was reported as ΦΔ =
0.47 ± 0.05, as derived from miniSOG’s ability to photooxidize
the fluorescent probe anthracene dipropionic acid (ADPA). In this
report, a significantly smaller value of ΦΔ = 0.03 ± 0.01 is obtained by two methods: direct measurement
of its phosphorescence at 1275 nm and chemical trapping using uric
acid as an alternative probe. We present insight into the photochemistry
of miniSOG and ascertain the reasons for the discrepancy in ΦΔ values. We find that miniSOG oxidizes ADPA by both
singlet oxygen-dependent and -independent processes. We also find
that cumulative irradiation of miniSOG increases its ΦΔ value ∼10-fold due to a photoinduced transformation of the
protein. This may be the reason why miniSOG outperforms other fluorescent
proteins reported to date as singlet oxygen generators.
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alternative probelight irradiationminiSOG increasessinglet oxygen generatorselectron microscopyphotoinduced transformationprobe anthracene dipropionic acidSinglet Oxygen GenerationGenetically Encoded Tag miniSOGThecorrelative lightminiSOG oxidizes ADPAtag miniSOGΦΔ valuessinglet oxygen1275 nmuric acid
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