Single-cell RNA-Seq of human primary lung and bronchial epithelium cells
datasetposted on 13.03.2020 by Soeren Lukassen, Robert Lorenz Chua, Timo Trefzer, Nicolas C. Kahn, Marc A. Schneider, Thomas Muley, Hauke Winter, Michael Meister, Carmen Veith, Agnes W. Boots, Bianca P. Hennig, Michael Kreuter, Christian Conrad, Roland Eils
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
This dataset contains count matrices and per-cells metadata tables for RNA sequencing of 39778 single nuclei from healthy primary lung samples of 12 lung adenocarcinoma patients as well as 17451 single human bronchiole epithelial cells from 4 donors. All samples were processed using the 10X Genomics Chromium platform with v2 chemistry and sequenced with one sample per lane on an Illumina HiSeq4000. Reads were aligned to the hg19 reference genome version 1.2.0 obtained from 10X Genomics. Data processing was performed using Seurat3.
The metadata table includes patient ID, sex, age, smoking status, and cell type, as well as QC statistics (number of genes, number of cells, ratio of mitochondrial reads).