SinRR’ positively influences the expression of PaLoC genes.

(A) Quantification of toxins in parent R20291 and the sinRR’ mutant complemented strains using toxins specific ELISA. The data shown are means ± standard errors of three replicates. Statistical significance was tested using one-way ANOVA, followed by Dunnett’s multiple comparisons test comparing values to the average of the parent with vector control (*** <0.0005 p-value). (B) Increased intracellular levels of c-di-GMP in the sinRR’ mutant. Statistical analysis was performed using two-tailed t-test (* <0.05 p-value).