Simulations of oxidative stress in model C.

<p>(<i>A</i>) The steady state flux through the cytosolic pentose phosphate pathway in model C as a function of the oxidative stress by varying the kinetic constant <i>k<sub>TOX</sub></i>. (<i>B</i>) Fluxes through the cytosolic PPP enzymes as a function of time upon sudden oxidative stress. During the whole time-course, <i>k<sub>TOX</sub></i> = 2 µl·min<sup>−1</sup> · mg protein<sup>−1</sup>. The system is removed from steady state at <i>t</i> = 0, by setting 99% of the NAD(P)H and trypanothione pools to the oxidized form. Shown is the relaxation of the cytosolic PPP fluxes. Solid lines indicate medians, shaded areas show interquartile ranges. Near identical results were obtained for model D (<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi.1003371.s008" target="_blank">Figure S5</a>).</p>