Silencing ncRNA genes based on proximal H3K4me3/DNaseI signatures.

A) Schematic representation of the ncRNA knockout strategy. To abrogate transcription a signature consisting of H3K4me3 and DNaseI hyper-sensitivity (DNase HSS) signals overlapping at the gene proximal promoter is excised through two flanking guideRNAs. B) Cloning strategy to obtain a pX458 CRISPR vector construct simultaneously expressing two guideRNAs driven by repeated U6 promoters for knockout of a given ncRNA gene TSS signature. The depicted insert is generated by gene synthesis.