Sensitivity of MN detection with PolyT length and dye variants.

Each of the indicated probes was combined with the various indicated concentrations of MN (diluted in buffer) and incubated at 37°C for 1 hour and then fluorescence was measured. Fluorescence values are normalized to those of control samples in which no nuclease was included. Fluorescence values for the lowest MN concentrations are also plotted with a different scale for better differentiation of the lower values (inset). Error bars indicate standard deviations of triplicate measurements.