jz7b01739_si_001.pdf (1.27 MB)
Selective Distance Measurements Using Triple Spin Labeling with Gd3+, Mn2+, and a Nitroxide
journal contribution
posted on 2017-10-09, 00:00 authored by Zuyan Wu, Akiva Feintuch, Alberto Collauto, Luke A. Adams, Luigi Aurelio, Bim Graham, Gottfried Otting, Daniella GoldfarbDistance
measurements by pulse electron paramagnetic resonance
techniques, such as double electron–electron resonance (DEER,
also called PELDOR), have become an established tool to explore structural
properties of biomacromolecules and their assemblies. In such measurements
a pair of spin labels provides a single distance constraint. Here
we show that by employing three different types of spin labels that
differ in their spectroscopic and spin dynamics properties it is possible
to extract three independent distances from a single sample. We demonstrate
this using the Antennapedia homeodomain orthogonally
labeled with Gd3+ and Mn2+ tags in complex with
its cognate DNA binding site labeled with a nitroxide.