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Selected genes dysregulated in NSCs as compared to homogenates of isolated hypothalami.

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posted on 2017-10-20, 17:37 authored by Kara R. Vogel, Garrett R. Ainslie, Erwin E. Jansen, Gajja S. Salomons, Jean-Baptiste Roullet, K. Michael Gibson

Neural stems cells were harvested following 24 h of culture in 6-well plates; hippocampi were dissected from P1 and P20 (postnatal day of life) aldh5a1+/+ (white boxes) and aldh5a1-/- (black boxes) mice. Shown are Gabrb3, the β3 subunit of the GABAB receptor, solute carriers (Slc) 1a2, 12a2 and 12a5, respectively, representing family members of the solute carrier organic acid transporter family, and tumor necrosis factor α (TNFα). Gene expression was measured via quantitative reverse transcription-polymerase chain reaction (RT-PCR). NSC expression levels were normalized to wild-type NSCs, and hippocampal extract results were normalized to P20 wild-type mice. Bars denote the mean ± SD of 3–4 biological replicates. ND, denotes that expression was not detectable. Note the developmental differences in the solute carrier transporters as a function of both tissue age and NSCs, underscoring a high degree of ontogeny. Conversely, up-regulation of the β3 subunit of the GABAB receptor was consistent regardless of tissue or age. Statistical analysis, Student’s two-tailed t test; *p<0.05; **p<0.01; ***p<0.001.

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