ac504555u_si_001.pdf (90.43 kB)
Selected Overtone Mobility Spectrometry
journal contribution
posted on 2015-05-19, 00:00 authored by Michael
A. Ewing, Christopher R.
P. Conant, Steven M. Zucker, Kent J. Griffith, David E. ClemmerA new
means of acquiring overtone mobility spectrometry (OMS) data
sets that allows distributions of ions for a prescribed overtone number
is described. In this approach, the drift fields applied to specific
OMS drift regions are varied to make it possible to select different
ions from a specific overtone that is resonant over a range of applied
frequencies. This is accomplished by applying different fields for
fixed ratios of time while scanning the applied frequency. The ability
to eliminate peaks from all but a single overtone region overcomes
a significant limitation associated with OMS analysis of unknowns,
especially in mixtures. Specifically, a priori knowledge
via selection of the overtone used to separate ions makes it possible
to directly determine ion mobilities for unknown species and collision
cross sections (assuming that the ion charge state is known). We refer
to this selection method of operation as selected overtone mobility
spectrometry (SOMS). A simple theoretical description of the SOMS
approach is provided. Simulations are carried out and discussed in
order to illustrate the advantages and disadvantages of SOMS compared
with traditional OMS. Finally, the SOMS method (and its distinction
from OMS) is demonstrated experimentally by examining a mixture of
peptides generated by enzymatic digestion of the equine cytochrome c with trypsin.