Seed-set under natural conditions

To estimate the number of ovules and the seeds per fruit under natural conditions at each site (ridge, slope, and valley), we examined the plants growing within and nearby the quadrats over 2 years (2010 and 2011). The total number of pin and thrum plants examined was 78 and 79 during 2010 and 170 and 157 during 2011, respectively. We collected several fruits from each plant (2.8 on average) during 2010, and one fruit per plant during 2011. The total number of fruits collected from the pin and thrum plants was 233 and 204 during 2010 and 170 and 157 during 2011, respectively. The fruits were collected just before dehiscence, and the number of seeds and undeveloped seeds or ovules were counted using a microscope.