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Scalable 3D printed molds for human tissue engineered skeletal muscle
journal contribution
posted on 2019-03-08, 14:06 authored by Andrew CapelAndrew Capel, Rowan Rimington, Jacob Fleming, Darren J. Player, Luke Baker, Mark Turner, Julia Jones, Neil MartinNeil Martin, Richard FergusonRichard Ferguson, Vivek Mudera, Mark LewisMark LewisTissue engineered skeletal muscle allows investigation of the cellular and molecular
mechanisms that regulate skeletal muscle pathology. The fabricated model must
resemble characteristics of in vivo tissue and incorporate cost-effective and high content
primary human tissue. Current models are limited by low throughput due to the
complexities associated with recruiting tissue donors, donor specific variations, as well
as cellular senescence associated with passaging. This research presents a method
using fused deposition modeling (FDM) and laser sintering (LS) 3D printing to generate
reproducible and scalable tissue engineered primary human muscle, possessing aligned
mature myotubes reminiscent of in vivo tissue. Many existing models are bespoke
causing variability when translated between laboratories. To this end, a scalable model
has been developed (25–500 µL construct volumes) allowing fabrication of mature
primary human skeletal muscle. This research provides a strategy to overcome limited
biopsy cell numbers, enabling high throughput screening of functional human tissue.
Funding
The authors would like to acknowledge Loughborough University, EPSRC (grant reference EP/L02067X/1) for funding and support for this work. In addition, this research was funded by the NIHR Leicester Biomedical Research Centre.
History
School
- Sport, Exercise and Health Sciences
Published in
Frontiers in Bioengineering and BiotechnologyVolume
7Citation
CAPEL, A.J. ... et al, 2019. Scalable 3D printed molds for human tissue engineered skeletal muscle. Frontiers in Bioengineering and Biotechnology, 7, Article 20.Publisher
Frontiers Media © The AuthorsVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/Acceptance date
2019-01-28Publication date
2019-02-14Notes
This is an Open Access article. It is published by Frontiers Media under the Creative Commons Attribution 4.0 International License (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/ISSN
2296-4185eISSN
2296-4185Publisher version
Language
- en