pone.0201669.g003.tif (2.17 MB)
SURFIN4.2 protein forms a complex with GLURP and RON4.
figure
posted on 2018-08-09, 17:42 authored by Maria del Pilar Quintana, Jun-Hong Ch’ng, Arash Zandian, Maryam Imam, Kjell Hultenby, Michael Theisen, Peter Nilsson, Ulrika Qundos, Kirsten Moll, Sherwin Chan, Mats WahlgrenIP with αSURFIN4.2 specifically pulls down SURFIN4.2 from schizont (A) and merozoite (B) protein extract as compared with control IgGs. (C) IP with αSURFIN4.2 also pulls down GLURP and RON4. (D) IP with αGLURP R2 also pulls down RON4 but not SURFIN4.2; IP with αRON4 also pulls down GLURP and SURFIN4.2. Input corresponds to the supernatant fraction after SB3-10 extraction followed by detergent removal and pre-clearing on control beads; FT: Flow-through corresponds to unbound material; E1 and 2: Eluted fraction corresponds to bound material to the antibody-coupled beads.
