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SR-B1 is dispensable for HCV entry into Huh7 cells.

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posted on 2016-05-06, 03:30 authored by Satomi Yamamoto, Takasuke Fukuhara, Chikako Ono, Kentaro Uemura, Yukako Kawachi, Mai Shiokawa, Hiroyuki Mori, Masami Wada, Ryoichi Shima, Toru Okamoto, Nobuhiko Hiraga, Ryosuke Suzuki, Kazuaki Chayama, Takaji Wakita, Yoshiharu Matsuura

(A) Expressions of CD81, SR-B1, CLDN1, and OCLN in parental, CD81 KO, SR-B1 KO, CLDN1 KO and OCLN KO Huh7 cells were determined by immunoblotting analysis. (B) Cells were infected with HCVcc at an MOI of 1, and intracellular HCV RNA levels at 24 h post-infection were determined by qRT-PCR. (C) Infectious titers in the supernatants at 72 h post-infection were determined by focus-forming assay. In all cases, asterisks indicate significant differences (*P<0.05; **P<0.01) versus the results for parental Huh7 cells. “n.d.” indicates HCV titer below 10 FFU/ml.

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