SMBE_poster_FINAL.pdf (1.14 MB)
RNAseq gene expression estimates altered by trimming parameters and choice of genome alignment
Version 3 2017-08-07, 22:28
Version 2 2017-08-07, 22:26
Version 1 2017-08-07, 22:22
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posted on 2017-08-07, 22:28 authored by Kimberly OlneyKimberly Olney, Sarah Brotman, Valeria Valverde-Vesling, Jocelyn Andrews, Melissa Wilson SayresMelissa Wilson SayresOne potential confounder of RNAseq analysis can result when applying a
standardized pipeline to samples of different sexes in species with chromosomal
sex determination. Because of the homology between the human X and Y
chromosome, we expect that mis-mapping will routinely occur between these two chromosomes,
artificially affecting estimates of sex-linked gene transcription. For this reason we tested alternative
alignment scenarios on RNAseq samples from the brains of 5 genetic female and 5
genetic males to assess how inferences of differential gene
expression patterns change depending on the reference genome.