figshare
Browse
SMBE_poster_FINAL.pdf (1.14 MB)

RNAseq gene expression estimates altered by trimming parameters and choice of genome alignment

Download (1.14 MB)
Version 3 2017-08-07, 22:28
Version 2 2017-08-07, 22:26
Version 1 2017-08-07, 22:22
figure
posted on 2017-08-07, 22:28 authored by Kimberly OlneyKimberly Olney, Sarah Brotman, Valeria Valverde-Vesling, Jocelyn Andrews, Melissa Wilson SayresMelissa Wilson Sayres
One potential confounder of RNAseq analysis can result when applying a standardized pipeline to samples of different sexes in species with chromosomal sex determination. Because of the homology between the human X and Y chromosome, we expect that mis-mapping will routinely occur between these two chromosomes, artificially affecting estimates of sex-linked gene transcription. For this reason we tested alternative alignment scenarios on RNAseq samples from the brains of 5 genetic female and 5 genetic males to assess how inferences of differential gene expression patterns change depending on the reference genome.

History

Usage metrics

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC