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SDS-PAGE analysis of recombinant S. plymuthica AS9 PalI and its variants expressed in E. coli BL21(DE3).

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posted on 2016-02-17, 11:47 authored by Xuguo Duan, Sheng Cheng, Yixin Ai, Jing Wu

(A) Lane M, molecular weight markers; Lane 1, culture supernatant of the wild-type enzyme; Sucrose isomerase is indicated by the black arrow. (B) Lane M, molecular weight markers; Lane 1, purified wild-type enzyme; Lane 2, purified E175N; Lane 3, purified K576D; Lane 3, purified E175N/K576D.

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    BLenzymeSerratia plymuthica Sucrose IsomeraseE 175N mutantsAS 9 PalISerratia plymuthica AS 9577KE 175NE 175N K 576D K 174D G 176D S 575DE 175N K 576DE 175N K 576D E 175NK 576D mutants

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