Romidepsin, Panobinostat, and PTC-209 impact histone modifications at the RTA promoter.

(A) BCBL-1 cells were treated with DMSO or 1 μM Romidepsin or Panobinostat for four hours, and levels of H3Ac and total H3 at the RTA promoter were determined by ChIP-qPCR. Levels of H3Ac were normalized to total levels of H3, and the fold difference between DMSO and compound treated cells was calculated. (B) BCBL-1 cells were treated with DMSO or 10 μM PTC-209 for five days, and levels of H2AK119ub and total H2A at the RTA promoter were determined by ChIP-qPCR. Levels of H2AK119ub were normalized to total levels of H2A, and the fold difference between DMSO and PTC-209 treated cells was calculated. Data are representative of three independent experiments.