Role of interspecies interactions in dual-species biofilms developed <i>in vitro</i> by uropathogens isolated from polymicrobial urinary catheter-associated bacteriuria

2016-09-19T22:19:42Z (GMT) by E. M. Galván C. Mateyca L. Ielpi
<p>Most catheter-associated urinary tract infections are polymicrobial. Here, uropathogen interactions in dual-species biofilms were studied. The dual-species associations selected based on their prevalence in clinical settings were <i>Klebsiella pneumoniae</i>–<i>Escherichia coli</i>, <i>E. coli</i>–<i>Enterococcus faecalis</i>, <i>K. pneumoniae</i>–<i>E. faecalis</i>, and <i>K. pneumoniae</i>–<i>Proteus mirabilis</i>. All species developed single-species biofilms in artificial urine. The ability of <i>K. pneumoniae</i> to form biofilms was not affected by <i>E. coli</i> or <i>E. faecalis</i> co-inoculation, but was impaired by <i>P. mirabilis</i>. Conversely, <i>P. mirabilis</i> established a biofilm when co-inoculated with <i>K. pneumoniae</i>. Additionally, <i>E. coli</i> persistence in biofilms was hampered by <i>K. pneumoniae</i> but not by <i>E. faecalis</i>. Interestingly, <i>E. coli</i>, but not <i>K. pneumoniae</i>, partially inhibited <i>E. faecalis</i> attachment to the surface and retarded biofilm development. The findings reveal bacterial interactions between uropathogens in dual-species biofilms ranged from affecting initial adhesion to outcompeting one bacterial species, depending on the identity of the partners involved.</p>