Rictor deficiency reduces the humoral immune response.

Flow cytometry analysis of folicular (FO) and marginal zone (MZ) B cells in spleen of wild-type (WT) and Rictor knockout (KO) mice (n = 8) immunized with 4-hydroxy-3-nitrophenylacetyl–keyhole limpet hemocyanin (NP-KLH) (A). The quantification of percentage (B) and number (C) of FO and MZ B cells in the spleen of WT and Rictor KO mice. Flow cytometry analysis of germinal center (GC) B cells in the spleen of WT and Rictor KO mice immunized with NP-KLH (D). The quantification of percentage and number of GC B cells in the spleen of WT and Rictor KO mice (E). Flow cytometry analysis of memory B (MBC), plasmablast (PBC), and plasma cell (PC) cells in the spleen of WT and Rictor KO mice immunized with NP-KLH (F and H). The quantification of percentage and number of MBC, PBC, and PC cells in the spleen of WT and Rictor KO mice (G, I, and J). Mean results from ELISA detecting NP-specific immunoglobulin M (IgM) and Immunoglobulin G1 (IgG1) from immunized WT and Rictor KO mice (n = 6) (K). T-test was used to do the statistics, **p < 0.001. The numerical data (for C, E, G, I, J, K, and L) can be found in S1 Data.