Rhodomyrtone (Rho) causes aberrant fluorescent membrane stains.
(A)B. subtilis 168 stained with the membrane dye FM5-95 and the DNA dye DAPI. Blue arrows indicate membrane patches induced by rhodomyrtone. (B) Quantification of cells showing FM5-95-stained membrane patches in untreated (green, n = 311) and rhodomyrtone-treated (blue, n = 351) cells. See also overview microscopy pictures in S7 and S8 Figs). (C) B. subtilis 168 stained with the fluid lipid dye DiIC12. Natural RIFs are marked with yellow arrows, rhodomyrtone-induced fluid lipid accumulations with blue arrows. (D) Quantification of membrane foci per cells in untreated (green, n = 91) and rhodomyrtone-treated (blue, n = 93) cells. See also overview microscopy pictures in S10 and S11 Figs. (E) Time lapse microscopy showing fusion of native RIFs into large fluid lipid accumulations. (F) FM5-95 stain of B. subtilis strain 4277 (ΔmreB Δmbl ΔmreBH). Blue arrows indicate membrane patches induced by rhodomyrtone. Scale bars 2 μm.