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Rhodomyrtone (Rho) causes aberrant fluorescent membrane stains.

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posted on 2018-02-16, 18:42 authored by Dennapa Saeloh, Varomyalin Tipmanee, Kin Ki Jim, Marien P. Dekker, Wilbert Bitter, Supayang P. Voravuthikunchai, Michaela Wenzel, Leendert W. Hamoen

(A)B. subtilis 168 stained with the membrane dye FM5-95 and the DNA dye DAPI. Blue arrows indicate membrane patches induced by rhodomyrtone. (B) Quantification of cells showing FM5-95-stained membrane patches in untreated (green, n = 311) and rhodomyrtone-treated (blue, n = 351) cells. See also overview microscopy pictures in S7 and S8 Figs). (C) B. subtilis 168 stained with the fluid lipid dye DiIC12. Natural RIFs are marked with yellow arrows, rhodomyrtone-induced fluid lipid accumulations with blue arrows. (D) Quantification of membrane foci per cells in untreated (green, n = 91) and rhodomyrtone-treated (blue, n = 93) cells. See also overview microscopy pictures in S10 and S11 Figs. (E) Time lapse microscopy showing fusion of native RIFs into large fluid lipid accumulations. (F) FM5-95 stain of B. subtilis strain 4277 (ΔmreB Δmbl ΔmreBH). Blue arrows indicate membrane patches induced by rhodomyrtone. Scale bars 2 μm.

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