Rescue of <i>sphe</i> mutant phenotype using Gal4/UAS system.

2017-12-06T18:41:47Z (GMT) by Jelena Patrnogic Vincent Leclerc
<p>(A) <i>drosomycin</i> expression 24 hours PI, normalized to <i>rp49</i>, after immune challenge with <i>E</i>. <i>faecalis</i> (<i>sphe</i><sup><b><i>Δ</i></b><i>49</i></sup> vs. <i>sphe</i><sup><b><i>Δ</i></b><i>49</i></sup>; <i>UAS-sphe</i> p<0.0103, <i>sphe</i><sup><b><i>Δ</i></b><i>104</i></sup> vs. <i>sphe</i><sup><b><i>Δ</i></b><i>104</i></sup>;<i>UAS-sphe</i> p<0.0229). Controls (crtl) are <i>w</i><sup><i>1118</i></sup> wild type flies. (B) Survival rate after immune challenge with <i>E</i>. <i>faecalis</i> (<i>sphe</i><sup><b><i>Δ</i></b><i>49</i></sup> vs. <i>sphe</i><sup><b><i>Δ</i></b><i>49</i></sup>; <i>UAS-sphe</i> p<0.0001, <i>sphe</i><sup><b><i>Δ</i></b><i>104</i></sup> vs. <i>sphe</i><sup><b><i>Δ</i></b><i>104</i></sup>;<i>UAS-sphe</i> p<0.0001). For over expression of <i>sphe</i> using <i>UAS-sphe</i> lines we used ubiquitous <i>Act5C>Gal4</i>.</p>