IJNST-2167-8685-S3-001.pdf (1.07 MB)
Removal of Heavy Metals by Indigenous Microorganisms and Identification of Gene Responsible for Remediation
journal contribution
posted on 2017-12-07, 03:43 authored by Jaya SharmaThe present study deals with the removal of heavy metals from solid waste disposal sites. The waste generated from the
small scale industrial operations such as grinding, milling, cutting, rubbing, painting, washing are collected and dumped in
open grounds. The waste containing heavy metals was found subjected to action of rain, sun and atmospheric conditions
prevailing in the environment. The characterization of waste was done and indigenous microorganisms were isolated and
developed as culture for remediation of selected heavy metals Fe, Cu, Cd. The microbial consortium was subjected to exposure
of metals at increasing concentrations separately in a minimal salt medium using incubator shaker under controlled
environmental conditions and potential microorganisms were identified for each of the selected heavy metals using 16SrRNA
technique and bioinformatic tools. Bioremediation of heavy metals have been carried out at selected concentrations
viz.25ppm, 50ppm and 100ppm indigenous potential microorganisms Klebsiallasp. MHF ENV III using shake flask incubation
method. The potential organism Klebsiallasp. MHF ENV III have been found effective for bioremediation of heavy
metals (Fe, Cu and Cd). Klebsiallasp. MHF ENV III being a potential microorganism for bioremediation of heavy metals has
been further studied for identification of gene responsible for bioremediation.