Relative ATP consumption rates and initial ATP concentration in NSCs.
ATP concentrations were detected with an ATP-sensitive luminescent probe and monitored for 65 minutes to evaluate the rate of ATP consumption (A; indicating relative concentrations at that time point). Initial relative ATP concentration (B) between genotype and treatment. Data represents the mean of 3 replicates and error bars represent SD. XL-765 was employed at 10 nM in vehicle (Veh; 0.01% DMSO) for 24 h. Data in Fig 4A were analyzed using two-way ANOVA for half-life, as well as effects of time and treatment/genotype. This revealed interaction (half-lives;t1/2 in min.) for ATP consumption that were not significantly different between mutant (starvation conditions, 58.6 [50.6–69.5] and XL-765, 56.0 [44.4–75.7]) and wild-type (starvation conditions, 56.0 [48.9–65.2] and XL-765, 58.6 [48.1–75.0]) following 24 hr of growth/treatment (F (36, 104) = 0.8699; p = ns). Conversely, effects of time and treatment/genotype were significant (time, F (12, 104) = 41.85, p<0.0001; treatment/genotype, F (3, 104) = 177.4; p<0.0001). Data for Fig 5B was evaluated with a one-way ANOVA and post hoc analysis (F (3, 12) = 56.19; p<0.0001. Abbreviations employed were as in Figs 3 and 4.