Relationship between SG formation and innate immune response.

<p>(A) HEK293T cells were transfected with 50 ng IFNβ-Luc reporter and 20 ng TK-Luc reporter for 12 h, then mock-infected or infected with HPIV3 (MOI = 1 or 10) for 24 h. Cells were harvested for a luciferase assay. (B) HEK293T cells were transfected with 50 ng IFNβ-Luc reporter and 20 ng TK-Luc reporter together with the indicated RNA samples for 24 h. Cells were harvested for a luciferase assay. (C) HEK293T cells were transfected with 50 ng IFNβ-Luc reporter and 20 ng TK-Luc reporter together with the indicated plasmid encoding eIF2α and eIF20078-S51A for 24 h, then mock-infected or infected with HPIV3 (MOI = 1) for another 24h. Cells were harvested for a luciferase assay. (D) HEK293T cells with or without G3BP knockdown were transfected with 50 ng IFNβ-Luc reporter and 20 ng TK-Luc reporter for 12 h, then mock-infected or infected with HPIV3 (MOI = 1) for 24 h. Cells were harvested for a luciferase assay. (E) HEK293T cells with or without G3BP knockdown were mock-infected or infected with HPIV3 (MOI = 1) for 24 h. Cells were harvested to extract the nuclei fraction and the cytosol fraction. Protein samples were analyzed via western blot using anti-IRF3, anti-G3BP, anti-HN, anti-GAPDH, and anti-LMNB1 antibodies. Data are represented as means±SD. Student’s t test: * P<0.05, ** P<0.01, *** P<0.001, ns = not significant.</p>