Recurrent hormone-binding domain truncated ESR1 amplifications in primary endometrial cancers suggest their implication in hormone independent growth. Supplementary Optical Dataset S1

2016-05-22T03:31:43Z (GMT) by Frederik Holst
<p>The optical dataset illustrates the <i>ESR1</i> FISH signal appearance by full size FISH photographs of a total of 28 out of 29 primary endometrial cancers that had gone on to metastasize and that had copy-number changes characterized by SNP GeneChips (tumors #1-17 and #19-29).</p> <p> The three color photographs show the nuclear <i>ESR1</i> FISH signals in 4µm conventional large section FFPE tissue slides. Shown are <i>ESR1</i> signals (green), CEN6 signals (orange) and nuclei (blue). </p> <p>Photographs are taken using an Olympus IX51 microscope at 100´ magnification, an Olympus XM10 digital camera (1,376 ´ 1,032 pixels) and Olympus cellSens imaging software.</p> <p> </p><p>Increased <i>ESR1</i> FISH signals are marked exemplarily in some cases (white arrows and edges). These could reach from two signals nearby each other, for gene duplication to several green dots.</p> <p>For all 28 tumors pictures of up to four different tissue areas (Fig. 01-04) are available, including up to four different z-layers (Z-Stack A-D) per area to illustrate the distribution of signals within the z-axis.</p>