Real-time PCR analysis of gluconeogenesis mRNA expression in the liver.

<p>Real-time-PCR analyses of G6Pase, PEPCK, IRS1, IRS2 and GSK3β mRNA expression were performed on cDNA derived from total RNA prepared from fasted liver tissue of (a) 3 and (b) 6 month-old male wildtype (WT, n = 8), aromatase knockout (KO, n = 8) and 2.5 µg/day 17β-estradiol-treated KO (KOE, n = 7) mice. Expression data from 7–8 samples per genotype are shown following normalization for cyclophilin mRNA expression, and presented from replicate analysis as the mean ± SD. <i>*p&lt;0.05,**p&lt;0.01</i> and <i>***p&lt;0.001</i> versus expression in age-matched WT samples and <i><sup>##</sup>p&lt;0.01, <sup>###</sup>p&lt;0.05</i> versus KO samples.</p>